The BD FACSCanto™ II is an easy-to-use 6-colour analyser with two lasers (Blue 488nm and Red 640nm), a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled, and this combined with trigon and octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength. The BD FACS Loader carousel accommodates up to forty 12×75-mm tubes and automatically loads them on the BD FACSCanto II system without operator intervention.
Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 10,000 events/second. We recommend a minimum sample volume of 250 μL.
- Annexin V assay
- Cell Cycle Analysis
- Multiplex bead assay for cytokines
FACSCantoII – Basic Operation Protocol
Staff-in-charge: Ms. YAU, Ka Long (Erica) | 3917 9443