The FACSMelody is a 9-colour high speed cell sorter with three lasers (Violet 405nm, Blue 488nm and Red 640nm), a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled cuvette and this combined with heptagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength.
The 100um nozzle makes this equipment ideal to sort most types of tissue. It is equipped with a versatile sort collection chamber that allows users to sort into up to 2-way 1.5mL or 5mL tubes as well as the ACDU unit which allows users to sort into 96- or 384-well plate. Cooling and heating for sort collection are available.
Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.2 μm. The maximum data acquisition rate is 70,000 events/second and maximum sorting rate is 8,250 events/second (at 33kHz). We recommend a minimum sample volume of 500 μL.
- Multi-color Cell Sorting (up to 9 colors)
- Index Sorting
- Single Cell Sorting
BD FACSMelody Cell Sorter – Basic Operation Procotol
Staff-in-charge: Ms. YAU, Ka Long (Erica) | 3917 9443