LSM780 is an inverted laser scanning confocal microscope. It is equipped with three solid state lasers (405, 561 and 633 nm), one argon laser with three laser lines (458, 488 and 514 nm), one femtosecond pulsed laser (Coherent Chameleon Vision II, tuning range 680nm-1080nm), two PMT detectors, one 32-channel spectral GaAsP detector and one transmitted light PMT detector. The GaASP detector allows the detection of weak signals and spectral imaging with unmixing (separation of spectrally overlapping fluorophores). The stage-top live cell chamber, the motorized stage, photo-bleaching module enables the multi-dimensional (multi-channel, multi-position, time-lapse) live cell imaging, photon-manipulation and large tissue tile scanning. Applications:

• XY 2D / Z-stack 3D imaging, DIC/Phase contrast
• Live cell imaging;
• Two-photon imaging;
• Spot/line Scan; lambda scan, linear unmixing;
• Tile scanning; multiple position imaging.
• Fluorescence recovery after photobleaching (FRAP), Fluorescence resonance energy transfer (FRET)

Detailed Configuration Standard Operation Protocol – Carl Zeiss LSM780 Staff-in-charge: Dr. CHEN, Miao | 3917 9806