Imaging and Flow Cytometry Core
LSM780 is an inverted laser scanning confocal microscope. It is equipped with three solid state lasers (405, 561 and 633 nm), one argon laser with three laser lines (458, 488 and 514 nm), one femtosecond pulsed laser (Coherent Chameleon Vision II, tuning range 680nm-1080nm), two PMT detectors, one 32-channel spectral GaAsP detector and one transmitted light PMT detector. The GaASP detector allows the detection of weak signals and spectral imaging with unmixing (separation of spectrally overlapping fluorophores). The stage-top live cell chamber, the motorized stage, photo-bleaching module enables the multi-dimensional (multi-channel, multi-position, time-lapse) live cell imaging, photon-manipulation and large tissue tile scanning. Applications:
LSM 800 is an inverted confocal setup equipped with 4 lasers (405 nm, 488 nm, 561 nm, 640nm) and Airyscan for sub-diffraction resolution to 140 nm. The microscope has 2 confocal detectors and a highly sensitive GaAsP detector, each of the detector’s wavelengths is tunable per nanometer. The stage-top live cell chamber, the motorized stage, advanced tiling module, photo-bleaching module enables the multi-dimensional (multi-channel, multi-position, time-lapse) live cell imaging, photo-manipulation and big tissue tile scanning. Applications:
The Zeiss LSM880 with Ariyscan 2 is an inverted confocal microscope. The system is equipped with 7 laser lines 405nm, 458nm, 488nm, 514 nm, 561nm, 594nm and 633 nm. The newly designed excitation beam is elongated in y which acquires four lines of image information instead of only one with just one horizontal scanner movement. It offers high sensitivity, enhanced resolution in x, y and z, and high image-acquisition speed in one system and achieve a 1.7× higher resolution in all spatial dimensions, 120 nm laterally and 350 nm axially which allows fast biological processes with minimal phototoxicity. The stage-top live cell chamber, the motorized stage, advanced tiling module, photo-bleaching module enables the multi-dimensional (multi-channel, multi-position, time-lapse) live cell imaging, photo-manipulation and big tissue tile scanning.
The LSM900 is an inverted confocal microscope with 4 solid state lasers (405nm, 488nm, 561nm, 640 nm) and Airyscan2 scanner for sub-diffraction resolution of 120 nm laterally and 350 nm axially. Airyscan2 in Multiplex mode can acquire up to four image lines with high SNR in a single sweep enabling unique combination of high speed, high resolution and high sensitivity. New ZEN software modules, including “Sample Navigator” and “Zen Connect” will simplify the imaging setup procedure. The stage-top live cell chamber, the motorized stage, advanced tiling module, photo-bleaching module enables the multi-dimensional (multi-channel, multi-position, time-lapse) live cell imaging, photo-manipulation and large tissue tile scanning.
The LSM900 inverted confocal microscope is equipped with four solid state lasers (405nm, 488nm, 561nm, 635 nm) and two PMT detectors. The motorized stage, advanced tiling module enables the multi-dimensional (multi-channel, multi-position) imaging and large tissue tile scanning.
Standard Operation Protocol – LSM 900 Inverted Confocal
Staff-in-charge: Ms. CHAN, Cherry | 3917 7556 | firstname.lastname@example.org
LSM900 upright confocal is an upright confocal microscope equipped with 4 lasers (405 nm, 488 nm, 561 nm, 640nm), two PMT detectors and one transmitted light detector. The motorized stage, advanced tiling module enables the multi-dimensional (multi-channel, multi-position, time-lapse) imaging and big tissue tile scanning. ZEN software modules, including “Sample Navigator” and “Zen Connect” will simplify the imaging setup procedure.
Standard Operation Protocol – LSM 900 Upright Confocal
Staff-in-charge: Ms. CHAN, Cherry | 3917 7556 | email@example.com
The Zeiss LSM980 system is an inverted laser scanning confocal microscopes with Airyscan 2 expands the excitation laser spot to allow imaging of eight lines in parallel simultaneously. It enables multiplex modes of SR-8Y, SR-4Y and CO-8Y for high speed imaging without sacrificing sensitivity or resolution. New ZEN software modules, including “Sample Navigator” and “Zen Connect” will simplify the imaging setup procedure. The stage-top live cell chamber, the motorized stage, advanced tiling module, photo-bleaching module enables the multi-dimensional (multi-channel, multi-position, time-lapse) live cell imaging, photo-manipulation and large tissue tile scanning.
Staff-in-charge:Ms ZHU, Hanqi | 3910-3995 | firstname.lastname@example.org
SRiS 2.0 is a set up build for performing stochastic optical reconstruction microscopy (STORM). Its unique design allows two-channel simultaneous acquisition in 656 nm and 750 nm channels. Active locking function in x-y axis and z-axis is maintained throughout imaging to achieve stability in nanometer scale. Applications:
Live-SR Super-resolution/TIRF Microscope is an integrated imaging system equipped with a Live-SR Super-resolution Unit, a Yokogawa CSU-W1 Spinning Disk Unit, and an iLAS3 Ring-TIRF/FRAP/Ablation Unit. The Live-SR is based on an optically demodulated structured illumination technique with online processing. It enables super-resolution (105nm laterally) at high speed with low photo-toxicity. iLAS3 Ring-TIRF/FRAP with 4 laser lines (405nm, 488nm, 561nm, and 639nm) allows the observation of membrane-associated processes like cell adhesion, hormone binding, molecule transport, exocytotic and endocytotic dynamics. A high-power 355nm UV pulsed laser enables ablation for applications like DNA damage and axotomy.
Standard Operation Protocol – Live-SR Super Resolution/TIRF Microscope
Staff-in-charge: Ms ZHU Hanqi | 3910 3995 | email@example.com
Vectra Polaris integrates both multispectral imaging and automated slide scanning to better visualize and quantify immunolabelled cells in tissue sections and tissue microarrays. With Tyramide signal amplification-based staining of multiple biomarkers (up to 8 overlapping fluorescent colors and DAPI), visualization is achieved by patented Liquid Crystal Tunable filter and unmixing algorithm. This fully automated system provides high speed whole-slide scanning at 10x to 40x in brightfield or fluorescence for up to 80 slides in a run. Supported by the tissue analysis software, quantification and spatial analysis of biomarkers can be accomplished in high throughput via machine learning and batch analysis.
IN Cell Analyzer 6500HS is a laser-based line scanning high-content imaging system. High speed filter based widefield, EDGE confocal imaging modes with a sensitive PCO Edge 4.2 sCMOS camera. User could choose between fixed cell imaging, live cell incubation and hypoxic incubation ([Oxygen] adjustable 0.1% -20%). Combined with new IN Carta software, for object quantification streamlines workflow for high content analysis feature extraction. Applications:
MACSima Imaging System is a widefield epifluorescence-based multiplex (up to 200 markers) imaging system equipped with liquid handling system and sCMOS camera for fixed tissue or cell sample imaging. It uses the principle of cyclic staining with different fluorochrome-conjugated antibodies (DAPI/FITC/PE/APC) to acquire microscopy data for a multitude of parameters. Combined with MACSima IQ view analysis software, quantification analysis of multiple biomarkers can be accomplished in high throughput via segmentation analysis.
Standard Operation Protocol – MACSima
Sample Prepration Protocols:
Olympus FVMPE-RS Hybrid Multiphoton System is a multi-photon laser scanning microscope that allows fluorescence imaging deep into specimens at hundreds of micrometers in living cells and tissues. It is equipped with two Coherent Chameleon Vision II laser, tunable from 680nm to 1080nm, four NDD (non-descanned detector) detectors (PMT), upright microscope stand and dual scanners (Galva and Resonant Scanner). This enables simultaneous imaging of maximum four fluorescent channels on live samples. High NA water immersion objectives allow imaging directly into specimens in media. It is dedicated to intravital imaging of living, whole mount, thickly sliced specimens or small animals.
Nikon Ti2-E is an inverted fluorescence microscope equipped with motorized and intelligent components for advanced imaging applications at high speed and high sensitivity. The integration of an unprecedented 25mm field of view with industry-leading optics, a Perfect-auto-Focusing System and a stratum structure enable the Nikon Ti2-E to have extra expandability meeting the needs for various type of imaging. Applications:
Standard Operation Protocol – Nikon Ti2-E Widefield Microscope
Staff-in-charge: Ms. CHAN Cherry | 3917 7556 | firstname.lastname@example.org
Color Imaging Microscope is an inverted microscope with high-speed CMOS color camera and fluorescent LED illumination. It is capable of brightfield color imaging and widefield fluorescent imaging. With high-speed color camera and multiple image alignment (MIA) function, it enables whole tissue color imaging acquisition by creating a real-time auto-stitched image when the specimen is focused and moved on the stage. The 2.3-megapixel camera’s CMOS sensor is combined with pixel-shifting technology, resulting in a 20.7-megapixel resolution. For live capturing, high-definition (HD) 1920 x 1200-pixel images can be captured at 60 fps, enabling clear observation and real-time focusing and acquisition with no image deterioration. Applications:
Standard Operation Protocol – Color Imaging Microscope
Staff-in-charge: Dr. WANG, Cindy | 3917 9851 | email@example.com
AxioZoom.V16 is a multi-zoom stereoscope with fluorescent illumination. High N.A. optics enable visualization of specimen ranging from large field-of-view to single cell observation. Flexible design allows illumination options of brightfield, dark field, oblique ring illumination, reflective fluorescence for DAPI / GFP / RFP. Applications:
Optical Tweezers/TIRF system is an integrated platform enables users to control, manipulate and observe samples in real time with nanometer precision and femtoNewton resolution. The NanoTracker™ 2 optical tweezers module enables trapping and tracking samples from several µm down to 30nm by using a highly focused 1064nm laser beam for sensitive manipulation, force, and tracking experiments. The iLAS2 ring-TIRF Unit with 4 laser lines (405nm, 488nm, 561nm, and 640nm) is also attached to this system for TIRF imaging.
Staff-in-charge: Ms ZHU, Hanqi | 3910-3995 |firstname.lastname@example.org
The Hong Kong Jockey Club Building
for Interdisciplinary Research
5 Sassoon Road
Pokfulam, Hong Kong
Mon-Fri: 9:00am – 5:30pm
Samples and goods reception not available 1:00pm – 2:00pm
Closed on Saturday, Sunday, all University and Public holidays.
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