MACSima Imaging System
Imaging Equipment
MACSima Imaging System is a widefield epifluorescence-based multiplex (up to 200 markers) imaging system equipped with liquid handling system and sCMOS camera for fixed tissue or cell sample imaging. It uses the principle of cyclic staining with different fluorochrome-conjugated antibodies (DAPI/FITC/PE/APC) to acquire microscopy data for a multitude of parameters. Combined with MACSima IQ view analysis software, quantification analysis of multiple biomarkers can be accomplished in high throughput via segmentation analysis.
Applications:
- Fluorescence imaging for fixed tissue section or fixed cells
- Multiplex imaging, quantification and analysis of tissue sections
Standard Operation Protocol – MACSima
Experiment Setup:
Sample Prepration Protocols:
MACSima sample preparation protocol_adherent cells
MACSima sample preparation protocol_frozen tissue
MACSima sample preparation protocol_FFPE tissue
How to setup a MACSwell Imaging Frame
Antibody test protocol for the use in the MACSima Imaging System
