Sample Quantitation – Qubit

Technology Overview

Qubit 2.0 Fluorometer

Accurate DNA and RNA quantitation is crucial for sensitive applications such as Next-generation sequencing (NGS). The fluorescent-based Qubit assay offers superior accuracy and unsurpassed sensitivity specific for quantifying a single analyte (i.e. DNA, RNA, or protein), compared to traditional UV-based measurement (e.g. Nanodrop). The fluorescent-based Qubit assay offers superior accuracy and unsurpassed sensitivity specific for quantifying a single analyte (i.e. DNA, RNA, or protein), compared to traditional UV-based measurement (e.g. Nanodrop).

^** CPOS Genomics Core no longer provides self-service for Qubit fluorometer. Please visit Imaging and Flow Cytometry Core for self-service measurement (Imaging and Flow Cytometry Core)

Sensitivity:

Detail information from Life Tech:

Service Charges and Ordering

Service Charges

Effective 1-Sep-2021

dsDNA HS Assay


Sample number	Full-Service*
	HKU Academic Research
	Bundle price	Per sample price
1 – 12	HK$ 330	N/A
13 – 30	N/A	HK$ 27
31 – 60		HK$ 25
61 – 100		HK$ 23
101 or more		HK$ 20

RNA HS Assay


Sample number	Full-Service*
	HKU Academic Research
	Bundle price	Per sample price
1 – 12	HK$ 350	N/A
13 – 30	N/A	HK$ 28
30 or more		HK$ 26

Remark ^*CPOS Genomics Core no longer provides self-service for Qubit fluorometer. Please visit Imaging and Flow Cytometry Core for self-service measurement For non-HKU academic research price, please enquire. Commercial clients welcome, please enquire. For academic institutes or commercial clients outside of Hong Kong, an extra HK$ 500 (per payment transaction) handling fee is required if the payment is made by bank draft or telegraphic transfer. *IMPORTANT:* To qualify for HKU pricing, the investigator must be a regular employee of HKU AND payment must be made from an internal HKU financial account that qualifies for internal transfer. Overhead charge is required for all incoming external funding.

Service Ordering

Please submit service request through iLab.

Workflow

Sample Preparation and Submission

General Guidelines

Submission of 12 or more samples is most cost effective.
Submit service request through iLab.
Fill in the Sample Submission Form (Excel) with sample information, include Nanodrop readings (if assayed before)
Submit Sample Submission Form as attachment in iLab.
Submit individually-numbered tubes (on ice /dry ice), in >=3 μl aliquots.
Please specify the buffer use.

Terms of Service

Processing Time

After sample submission, results will be delivered electronically (in an excel file) within 1-2 working days.

Invoice will be sent on the following month after completion of job.

Leftover Samples

After the completion of the experiment, samples will be kept for 1 week.

Users are welcome to collect any leftover materials during this period, after which, they will be discarded without notification.

Please contact platform specialist for pick-up arrangement.

Contact

Mr YIU, Francis

2831-5477
qubit.cpos@hku.hk

Sample Quantitation – Qubit

Technology Overview

Qubit 2.0 Fluorometer

Service Charges and Ordering

Service Charges

Service Ordering

Workflow

Sample Preparation and Submission

General Guidelines

Terms of Service

Processing Time

Leftover Samples

Contact

2831-5477

qubit.cpos@hku.hk

LKS Faculty of Medicine, HKU

Core Facilities

Address

Office Hours