Technology Overview
Qubit 2.0 Fluorometer
Accurate DNA and RNA quantitation is crucial for sensitive applications such as Next-generation sequencing (NGS). The fluorescent-based Qubit assay offers superior accuracy and unsurpassed sensitivity specific for quantifying a single analyte (i.e. DNA, RNA, or protein), compared to traditional UV-based measurement (e.g. Nanodrop). The fluorescent-based Qubit assay offers superior accuracy and unsurpassed sensitivity specific for quantifying a single analyte (i.e. DNA, RNA, or protein), compared to traditional UV-based measurement (e.g. Nanodrop). ** CPOS Genomics Core no longer provides self-service for Qubit fluorometer. Please visit Imaging and Flow Cytometry Core for self-service measurement (Imaging and Flow Cytometry Core) Sensitivity: 
Detail information from Life Tech:
Service Charges and Ordering
Service Charges
Effective 1-Sep-2021
dsDNA HS Assay
| Sample number | Full-Service* | |
| HKU Academic Research | ||
| Bundle price | Per sample price | |
| 1 – 12 | HK$ 330 | N/A |
| 13 – 30 | N/A | HK$ 27 |
| 31 – 60 | HK$ 25 | |
| 61 – 100 | HK$ 23 | |
| 101 or more | HK$ 20 |
| Sample number | Full-Service* | |
| HKU Academic Research | ||
| Bundle price | Per sample price | |
| 1 – 12 | HK$ 350 | N/A |
| 13 – 30 | N/A | HK$ 28 |
| 30 or more | HK$ 26 |
For other academic researchers and commercial clients, please enquire.
*IMPORTANT:* To qualify for HKU pricing, the investigator must be a regular employee of HKU AND payment must be made from an internal HKU financial account that qualifies for internal transfer. Overhead charge is required for all incoming external funding.
Service Ordering
Please submit service request through iLab.
Workflow
Sample Preparation and Submission
General Guidelines
- Submission of 12 or more samples is most cost effective.
- Submit service request through iLab.
- Fill in the Sample Submission Form (Excel) with sample information, include Nanodrop readings (if assayed before)
- Submit Sample Submission Form as attachment in iLab.
- Submit individually-numbered tubes (on ice /dry ice), in >=3 μl aliquots.
- Please specify the buffer use.
Terms of Service
Processing Time
After sample submission, results will be delivered electronically (in an excel file) within 1-2 working days.
Invoice will be sent on the following month after completion of job.
Leftover Samples
After the completion of the experiment, samples will be kept for 1 week.
Users are welcome to collect any leftover materials during this period, after which, they will be discarded without notification.
Please contact platform specialist for pick-up arrangement.
Contact
Mr YIU, Francis
