Vectra Polaris integrates both multispectral imaging and automated slide scanning to better visualize and quantify immunolabelled cells in tissue sections and tissue microarrays. With Tyramide signal amplification-based staining of multiple biomarkers (up to 8 overlapping fluorescent colors and DAPI), visualization is achieved by patented Liquid Crystal Tunable filter and unmixing algorithm. This fully automated system provides high speed whole-slide scanning at 10x to 40x in brightfield or fluorescence for up to 80 slides in a run. Supported by the tissue analysis software, quantification and spatial analysis of biomarkers can be accomplished in high throughput via machine learning and batch analysis. Applications:

• Brightfield microscopy of histological sections (e.g. DAB, H&E)
• Whole-slide widefield fluorescent scanning (e.g. in-vitro GFP expression)
• Multispectral imaging, quantification and analysis of immunolabelled sections (up to 8 fluorescent colors and DAPI)

Detailed Configuration Standard Operation Protocol – Perkinelmer Vectra Polaris Opal staining protocol Staff-in-charge: Mr. LEUNG, Ho Chi | 3917 9442