Analytical Instruments
Bioresearch Support Core
Analytical instruments
Bio-Rad QX200 Droplet Digital PCR System
Bio-Rad QX200 Droplet Digital PCR System
The QX200 allows extensive partitioning of nucleic acid samples into thousands of nanoliter-sized droplets, followed by well controlled PCR amplification and accurate end-point data acquisition. This third generation PCR technology enabls absolute quantification of target DNA copies per input sample without the need for running standard curves as well as eiminating error due to suboptimal amplification efficiency. It is highly sensitive and accurate to detect of rare (mutation) targets and enable precise copy number variation determination. Applications:
- Absolute quantification of target gene expression
- Rare mutation/ variant detection
- Copy Number Variation studies
- Pathogens load detection
Detailed configuration – QX200 ddPCR System
Standard Operation Protocol – QX200 ddPCR System
Location: L6-01 & L6-03, Laboratory Block, 21 Sassoon Road
Staff-in-charge: Ms CHEN, Wendy | 3917 9442 | bioresearch.cpos@hku.hk
Roche LightCycler480 II Real-time PCR
Agilent Seahorse XFe96 Analyzer
BMG CLARIOstar Plus
BMG CLARIOstar PlusCytiva Amersham Typhoon5 Biomolecular Imager
Cytiva Amersham Typhoon5 Biomolecular ImagerCytiva Amersham Imager 680 Blot and Gel Imager
Cytiva Amersham Imager 680 Blot and Gel ImagerThermoFisher Qubit Fluorometer
ThermoFisher Qubit FluorometerEdinburgh Instrument FS5
Extended services
Bruker AVANCE III 600 MHz NMR Spectrometer
Bruker AVANCE III 600 MHz NMR Spectrometer
(Service provided by the Department of Microbiology)
Bruker Avance III 600 MHz NMR Spectrometer is a high-resolution solution Nuclear Magnetic Resonance system equipped with a high sensitivity broad band inverse probe. Coupling with a SampleXpress autosampler and state-of-art TopSpin 3.1 software, the system offers fully automated sample loading and NMR data collection to maximize samples throughput. Over 300 1D, 2D and 3D NMR pulse programs are implemented with the system to allow wide range applications such as:
- Small molecule qualitative and quantitative analysis
- Structure determination of organic molecules and small peptides
- Small molecule sample or mixture purity determination
- Metabolomics studies on urine, serum, tissue and cell samples
- Ligand-observed NMR (WaterLogsy and STD-NMR) experiments for drug library screening and drug target identification and characterization
Detailed configuration – AVANCE 600 NMR
Location: L6-27, Laboratory Block, 21 Sassoon Road
Staff-in-charge: Mr. LAI Pok Man Ivan (Microbiology)| mednmr@hku.hk | 3917 9023 |
For sample submission, please fill in the online sample submission form
Spectrometer usage charge and manpower for sample preparation: $300/hr
(***Notice: service charge will be changed to $400/hr for sample submitted on or after 1 Sept 2022***)
IMPORTANT! PERSONS WITH CARDIAC OR OTHER MEDICALLY ACTIVE IMPLANTS, E.G. HEART PACEMAKER, MUST NOT ENTER L6-27.
THE SUPERCONDUCTING MAGNET MAY ALSO CAUSE PERMANENT DAMAGE TO MECHANICAL WATCH, MAGNETIC TAPES AND DISCS, AND MAGNETIC CREDIT CARD
