Bioresearch Support Core
(Service provided by the Department of Microbiology)
Bruker Avance III 600 MHz NMR Spectrometer is a high-resolution solution Nuclear Magnetic Resonance system equipped with a high sensitivity broad band inverse probe. Coupling with a SampleXpress autosampler and state-of-art TopSpin 3.1 software, the system offers fully automated sample loading and NMR data collection to maximize samples throughput. Over 300 1D, 2D and 3D NMR pulse programs are implemented with the system to allow wide range applications such as:
Location: L6-27, Laboratory Block, 21 Sassoon Road
Staff-in-charge: Mr. LAI Pok Man Ivan (Microbiology)| firstname.lastname@example.org | 3917 9023 |
For sample submission, please fill in the online sample submission form
Spectrometer usage charge and manpower for sample preparation: $300/hr
(***Notice: service charge will be changed to $400/hr for sample submitted on or after 1 Sept 2022***)
IMPORTANT! PERSONS WITH CARDIAC OR OTHER MEDICALLY ACTIVE IMPLANTS, E.G. HEART PACEMAKER, MUST NOT ENTER L6-27.
THE SUPERCONDUCTING MAGNET MAY ALSO CAUSE PERMANENT DAMAGE TO MECHANICAL WATCH, MAGNETIC TAPES AND DISCS, AND MAGNETIC CREDIT CARD
The QX200 allows extensive partitioning of nucleic acid samples into thousands of nanoliter-sized droplets, followed by well controlled PCR amplification and accurate end-point data acquisition. This third generation PCR technology enabls absolute quantification of target DNA copies per input sample without the need for running standard curves as well as eiminating error due to suboptimal amplification efficiency. It is highly sensitive and accurate to detect of rare (mutation) targets and enable precise copy number variation determination. Applications:
Roche LightCycler480 includes interchangeable 96-well & 384-well thermal block for high throughput real-time PCR experiment. Built-in Therma-Base technology provides rapid and accurate temperature adjustment with exceptional well-to-well temperature homogeneity. Advanced optical system allows robust and accurate simultaneous capturing of all data from the wells. Applications:
Location: L6-01, Laboratory Block, 21 Sassoon Road
An automated capillary electrophoresis system with high sensitivity and resolution detection.
Fragment Analyzer is a high throughput nucleic acid analysis system to qualify and quantify a wide range of samples including cDNA, NGS library, PCR Fragment, Genomic DNA, total RNA etc. With automated samples intake as well as running 12 parallel capillaries electrophoresis and data acquisition at the same time, the Fragment Analyzer greatly accelerates the nucleic acid analysis in molecular workflow by processing maximum of 192 samples in a single operation. With improved sensitivities and resolution in detection, the analysis software provides accurate and detailed results on nucleic acid’s size distribution, concentration and quality for downstream processes.
A metabolic analyzer to measure and interrogate mitochrondrial oxidative respiration and cellular glycolysis.
Using patented label-free detection technology to measure Oxygen Consumption Rate and Extracellular Acidification Rate in living cells, the Agilent Seahorse XF Analyzer enables quick and easy real-time evaluation of cellular bioenergetic activities to allow better understanding of metabolism in normal cells as well as in wide variety of diseases. Seahorse XFe96 Analyzer allows high throughput assay in 96-well format for efficient study of complicated experiment, including extensive cells/treatments comparison or detailed dose-response analysis.
A Multifunction plate reader with advance option and comprehensive accessories. High End Multi-Mode Microplate reader with high efficiency Linear Variable Filter (LVF) Monochromators™, 2 Reagent injectors, and Atmospheric Control Unit. With advance option in detection parameters, component movement (focusing, sample mixing) and incubation control, it can meet most of the needs in nowadays high-throughput cell-based assay.
A 4-in-1 multifunctional image scanner with 5 high power lasers and photomultiplier tubes (PMT)
Typhoon5 is a multifunctional image scanner with 5 high power lasers is designed for versatile imaging and precise quantitation of fluorescent, colorimetric, and radiolabelled proteins and nucleic acids. Signals captured by highly sensitivity photomultiplier tubes, Typhoon5 is having detection limit of as low as 3pg protein. Typhoon5 allows high throughput scanning of up to 20gels/membranes & 9-multi-well pates. Multiple stages are also available to accommodate various types of samples (animal tissue, gel, membrane, plate, dish, slide, phosphor screen…). Applications:
Location: L2-01, Laboratory Block, 21 Sassoon Road
Sensitive Imager for chemiluminescence & colorimetric signal capture.
AI680 is equipped with Peltier cooled Fujifilm Super CCD & superior FujinonTM lens which allow high sensitivity and high-quality imaging of chemiluminescence signal from Western blot. With inbuilt analysis software, intensity and molecular weight analysis can be measured instantly.
A versatile imager for caturing and quantifing signal in daily gel and blot assay Quick, high-resolution gel and blot imaging for qualitative and quantitative nucleic acid analysis. Universal hood with built-in white epi-illumination and UV transillumination designed to capture fluorescent and chemiluminescent images without using a photographic darkroom.
Qubit offers highly sensitive and accurate biomolecule quantification at orders of magnitude more sensitive than UV absorbance. Fluorescent-based detection measure only fluorescent dyes bound to the target molecules, even at low concentrations.
The Hong Kong Jockey Club Building
for Interdisciplinary Research
5 Sassoon Road
Pokfulam, Hong Kong
Mon-Fri: 9:00am – 5:30pm
Samples and goods reception not available 1:00pm – 2:00pm
Closed on Saturday, Sunday, all University and Public holidays.
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