Use 200uL microtubes (sample less than 8) OR 8-well strip (sample less than 32)
Service Charges and Ordering
*IMPORTANT:* To qualify for HKU pricing, the investigator must be a regular employee of HKU AND payment must be made from an internal HKU financial account that qualifies for internal transfer. Overhead charge is required for all incoming external funding.
Note: Above prices are for in-person sample submission or using our sample collection box only.
For sample submission via courier delivery, users should make their own courier arrangements.
Courier delivery option is NOT AVAILABLE for cycle sequencing products.
For commercial clients, please enquire.
Please submit service request through iLab.
DOs and DON’Ts for Sample Submission
Sample Collection Boxes
There are three off-site DNA sequencing sample collection boxes installed for your convenience.
The CPOS offers daily sample pick-up service from the three sample collection boxes.
While CPOS will try to ensure sample security by providing boxes with locks, we shall not be responsible for any sample losses.
If your samples are precious, we recommend bringing them to our counter in person during office hours.
Do NOT drop off samples after pick-up time on Fridays, Saturdays and the day before holidays.
Sample Submission by Courier Delivery
Local non-HKU academic users should arrange courier delivery for sample submission by themselves. Please refer to below for details of the receiver’s information:
- Contact Person: CPOS
- Contact number: 2831-5500
- Address: 6F, HKJCBIR, 5 Sassoon Road, Pokfulam, HK
- Company Name: Centre for PanorOmic Sciences (CPOS)
Payment Method: Sender Paid (寄付)
Seal the strip/plate with strip caps.
Pack the samples in a sealed envelope.
Note: CPOS should not be held liable for any loss or damage of sample during delivery.
DNA Sequencing results are delivered to users as zipped file through Online Data Delivery System (ODDS), notification will be sent to the email provided on the service request form upon data delivery.
Our current turn-around time is 1-3 working days after sample submission. However, the turn-around time could be longer at times due to unforeseeable circumstances and busy sequencing schedules.
We recommend Sequencing Analysis Software for viewing the electropherograms, and SeqScape Software to perform sequence comparisons on variant identification, SNP discovery, and SNP validation.
Both software are available on our user data analysis workstations (User PC#2) in Room 6-01 at the Centre for PanorOmic Sciences (CPOS).
Users may make advanced bookings for the workstations via our online resource booking system (ORBS) or at our reception counter (6-02).
Alternatively, Sequence Scanner Software can be freely downloaded from ABI website. It is also a great tool for reviewing sequencing data.
DNA Sequencing Analysis using the Sequencing Analysis Software from ABI
Terms of Service
At CPOS, positive and negative controls are included for every step in every run to monitor cycle sequencing, purification and electrophoresis quality.
Data from every run are reviewed and results will only be released to clients if all controls passed in-house QC.
Please refer to “Data QC” page for the rationale, charging scheme and details for rerun request.
The “Troubleshooting” page will help you evaluate the need for rerun.
CPOS can provide consultations including a suitable protocol that may improve sequencing results.
Our goal is to give you the highest quality of sequencing data possible.
According to our sequencer manufacturer, there exists up to 10% sporadic instrument-associated failure due to:
- random bad/insufficient injection
- bubble in the capillary during polymer filling
- cross-capillary fluorescence due to the nature of capillary sequencing and the proximity of the capillaries to each other (~5um) in the detection cell of the 3730xl platform
CPOS offers complimentary default reruns for sequencing samples with sporadic instrument-associated failures or sub-optimal data.
Users could also request for rerun of the samples. However, for samples with strong intensity, the charging scheme is shown below:
The rescue criteria are defined as:
- CRL improved by ≥ 30%, with an absolute increase of 50bp, OR
- Overall TS improved by at least 5, with a value at least 35
CRL: Contiguous Read Length is the longest uninterrupted stretch of bases with quality higher than 20.
In the evaluation of the quality of each base, not only the quality value of individual base is used, but also those of adjacent bases within a window size of 20.
TS: Trace Score is the average basecall quality value of bases in the post-trim sequence.
Users should review their own data and exercise discretion before requesting reruns.
The “Troubleshooting” guide will offer assistance in making an informed decision.
Users are also welcome to consult our platform specialists on difficult cases.
CPOS reserves the rights for final discretion over borderline cases.
Always check raw signals and sequence tracing when evaluating sequencing results.
The following images were generated by ABI Sequence Scanner, which is available for free download on ABI website.
Click here for software installation instructions and operations.