Protocols

Imaging and Flow Cytometry Core

Imaging

Instrument Operation

  1. Basic Operation – Carl Zeiss LSM780
  1. Carl Zeiss LSM800
  1. Carl Zeiss LSM880
  1. Carl Zeiss LSM900
  1. Carl Zeiss LSM980
  1. Nikon Ti2-E Widefield Microscope
  1. PE UltraVIEW VoX Spinning Disk confocal Microscope
  1. PE Vectra Polaris
  1. Total Internal Reflection Fluorescent (TIRF) Microscope
  1. Olympus FVMPE-RS Hybrid Multiphoton System
  1. NBI SRiS STORM Super-Resolution Microscope
  1. Elyra Super-Resolution Microscope
  1. GE IN Cell Analyzer 6500HS
  1. Color Imaging Microscope
  1. Carl Zeiss AxioZoom.V16

Image Processing

  1. MetaMorph analysis software
  1. Imaris analysis software (Visualization)
  1. Zen Efficient Navigation (ZEN)
  1. Basic Operation – Zen Efficient Navigation (ZEN) Blue Edition
  1. Basic Operation – Volocity Image Visualization software

Miscellaneous

  1. How to chose the right microscope for your application?
  1. How to set up successful live cell imaging experiments?
  1. Protocol – immunostaining protocol cell culture
  1. Sample Preparation Guideline – NBI SRiS STORM Super-Resolution Microscope
  1. In Vivo Imaging Preparation

Flow Cytometry

Instrument Operation

Miscellaneous

  1. Guideline of choosing a suitable flow system for your experiment
  2. FlowJo Analysis Software – Basic Operation
  3. Useful Tips for Cell Sorting
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