Bruker timsTOF Pro combines ion mobility with quadrupole and Time of Flight (QTOF) technology
It has 2 unique separation and sequencing technologies – Trapped Ion Mobility Spectrometry (TIMS) and Parallel Accumulation Serial Fragmentation (PASEF)
TIMS is a separation technique in gas phase, which resolves sample complexity with additional dimension of separation on top of liquid chromatography (LC) and mass spectrometry (MS). Ions are accumulated and released dependent on their mobility
With PASEF technology >120 Hz sequencing speed can be achieved with a duty cycle nearly without ion loss to increase sensitivity, peak capacity and confidence in compound characterization
Applications: shotgun proteomics, glycoproteomics, phosphoproteomics and lipidomics
Mass analyzing range 20 – 40,000
Mass accuracy with internal calibrant: better than 800ppb RMS Error
Resolution of 50,000 FSR (full sensitivity resolution) (@ 1222 m/z)
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