Flow Cytometry Equipment
Imaging and Flow Cytometry Core
Cell Sorter
BD FACSAria SORP
The BD FACSAriaTM SORP Cell Sorter is equipped with five lasers (Ultra-Violet 355nm, Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 640nm) which enables the detection of up to 18 fluorochromes. A gel coupled quartz cuvette flow cell in true fixed alignment with the laser, combined with trigon and octagon detection systems provide increased sensitivity and resolution.
A choice of nozzles (70, 86, 100 and 130 μm) allows optimization to a wide range of particle sizes. It is equipped with a versatile sort collection chamber that allows users to sort into up to 4-way 1.5- or 5-mL tubes, up to 2-way 15 mL tubes, as well as the ACDU unit which allows users to sort into 6-, 24-, 96-, 384-well plate or any custom collection device. Cooling and heating for sort collection are available.
Forward and side scatter properties allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 70,000 events/second and maximum sorting rate can go up to 7,000 events/second (at 28kHz). We recommend a minimum sample volume of 500 μL.
Applications:
- Multi-color Cell Sorting (up to 18 colors)
- Index Sorting
- Single Cell Sorting
Detailed Configuration
FACSAria SORP – Basic Operation Procotol
Staff-in-charge: Mr. Lau, Yin (Percy) | 3917 9443 | flow.cpos@hku.hk
BD FACSAria Fusion
The BD Influx with BSC
The BD Influx™ cell sorter is a 14-colour high speed cell sorter with four lasersBD Influx
The BD Influx™ cell sorter is a 16-colour high speed cell sorter with four lasers (Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 640nm), an adjustable optical alignment and a pressure-driven fluidics system. It is a jet-in-air system and the nozzle assembly is designed to produce high droplet frequencies at relatively low pressures. This allows high-speed sorting while maintaining cell viability and functionality.Cell Analyzer
BD LSR Fortessa
The BD LSRFortessa™ is a 17-colour analyser with four lasers (Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 640nm), a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled, and this combined with trigon and octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength, enabling researchers to identify cells with especially dim expression, or quantitate rare cell populations.
Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 40,000 events/second. We recommend a minimum sample volume of 400 μL.
Applications:
- Annexin V assay
- Cell Cycle Analysis
- Cell Proliferation assay
- Multiplex bead assay for cytokines
- Immunophenotyping
Detailed Configuration
LSR Fortessa – Basic Operation Protocol (Tube Mode)
LSR Fortessa – Basic Operation Protocol (HTS Mode)
Staff-in-charge: Mr. Lau, Yin (Percy) | 3917 9443 | flow.cpos@hku.hk
Agilent NovoCyte Quanteon
Agilent NovoCyte Quanteon analyzer is equipped with four lasers Agilent NovoCyte Advanteon BVR
The Agilent NovoCyte Advanteon BVYG
The BD FACSymphony A5 SE
