PacBio sequencing is a high throughput / third-generation sequencing technology which also known as single molecule real time (SMRT) sequencing. It utilizes zero-mode waveguides (ZMWs) to capture the real-time incorporation of nucleotides by DNA polymerase. This technology consistently produces long reads (average > 10,000 bp) which enables assembly of high-quality de novo genomes. It is also good for the survey of full-length transcript isoforms. Barcoding system is available for multiplexed sequencing on a single SMRT cell to achieve time- and cost- effectiveness.
PacBio platform service arrangement notice
If you are interested in PacBio sequencing, we continue to offer this service through outsourcing to a partner laboratory for library preparation and sequencing.
Please contact Dr Hin Kwok (hinkwok.cpos@hku.hk / 2831-5483) or platform specialists for project discussion.