Technology Overview
The Agilent 2100 Bioanalyzer is a microfluidics-based platform providing solutions for the analysis of DNA, RNA, proteins and cells with automated, high quality digital data output.
Bioanalyzer assays are suitable for various workflows, including next-generation sequencing (NGS), gene expression and genome editing. It is often adopted in routine RNA quality assessment and NGS sample quality check. As compared to traditional gel electrophoresis, the Bioanalyzer system has the added advantage of reduced sample consumption and less manual data processing.
While the assays can provide precise information on the size distributions of fragments, we DO NOT recommend them for accurate quantification of sample concentration. Instead, we suggest referring to fluorescent dye methods such as Qubit or PicoGreen assay for precise measurements of DNA or RNA concentration.
Service Charges and Ordering
Service Charges
Effective 1-Sep-2022
| Assay Type | Maximum Number of Samples per Chip | HKU Academic Research | Other Academic Research | ||
| per Sample | per Chip | per Sample | per Chip | ||
| RNA 6000 Nano | 12 | HK$ 67 | HK$ 800 | HK$ 84 | HK$ 1,000 |
| DNA 12000 | 12 | – | HK$ 710 | – | HK$ 910 |
| DNA High Sensitivity | 11 | HK$ 92 | HK$ 1,010 | – | HK$ 1,265 |
For commercial clients, please enquire.
*IMPORTANT*: To qualify for HKU pricing, the investigator must be a regular employee of HKU AND payment must be made from an internal HKU financial account that qualifies for internal transfer. Overhead charge is required for all incoming external funding.
Service Ordering
Please submit service request through iLab.
Workflow
Sample Preparation and Submission
The Core only keeps a small amount of stock of the RNA 6000 Nano chip, DNA 12000 chip and High Sensitivity DNA chip. For requests for more than 5 chips and/or for assays not listed below, please contact platform specialists at least three weeks in advance to make order arrangements.
Sample Preparation and Submission Guidelines
(1) Prepare samples in 0.5 mL tubes according to the qualitative range and buffer strength below. According to our in-house experience, concentration at approximately mid-way within the qualitative range gives best result. Concentration towards the low end may not yield reliable data.
| Bioanalyzer Assay | Sizing Range | Qualitative Range | Quantitative Range | Maximum Buffer Strength | Minimum Sample Volume | Number of Samples per Chip |
| RNA 6000 Nano total RNA# | 25 – 6000 nt | 5 – 500 ng/μL | 25 – 500 ng/μL | 100 mM Tris 0.1 mM EDTA or 125 mM NaCl 15 mM MgCl2 | 3 μL | 12 |
RNA 6000 Nano mRNA#| 25 – 6000 nt | 5 – 250 ng/μL | 25 – 250 ng/μL | 100 mM Tris | 0.1 mM EDTA or 125 mM NaCl 15 mM MgCl2 3 μL | 12 | |
| DNA 12000# | 100 – 12000 bp | – | 0.5 – 50 ng/μL* | 250 mM KCl 250 mM NaCl 15 mM MgCl2 | 3 μL | 12 |
| High Sensitivity DNA | 50 – 7000 bp | – | 5 – 500 pg/μL* | 10 mM Tris and 1 mM EDTA | 3 μL | 11 |
#Users are required to commit a whole chip for RNA 6000 Nano Assays for Prokaryotic RNA samples, RNA 6000 Nano mRNA Assay and DNA 12000 Assay.
*For NGS library QC, 30 – 40 ng/μL is needed for DNA 12000 Assay and 2 – 10 ng/μL is needed for High Sensitivity DNA Assay.
(2) Clearly label each tube with sample ID.
(3) Submit service request through iLab. Smear analysis from 200 bp to 1,000 bp would be provided for all DNA assays. Please specify if other ranges are needed.
(4) Submit Bioanalyzer sample submission form as attachment in iLab.
(5) Contact platform specialist for sample submission appointment.
(6) Bring the samples to the Bioanalyzer unit at the Core.
Special notes for Illumina Sequencing (Next Generation Sequencing) users
Please contact our Illumina platform specialists (nextgenseq.cpos@hku.hk) prior to submitting your samples to the Core.
*IMPORTANT*: It is not recommended to rely on the concentration indicated on the Bioanalyzer report for NGS library quantity check and NGS sequencing preparation. We advise using qPCR for accurate measurement of NGS library concentration.
For more details about submission requirements for Illumina Sequencing, kindly visit the Illumina Sequencing Sample Requirements and Submission webpage.
Data Collection
Processing Time
The expected turnaround time for a full Bioanalyzer chip analysis is usually 1-2 working days. Requests with sample number less than a full chip will be put into a job queue until enough samples are consolidated for a full chip analysis. As such, the turnaround time will vary depending on the number of samples collected.
Data Delivery
Results will be delivered in PDF format via our Online Data Delivery System (ODDS).
Unless otherwise specified, a smear analysis between 200bp to 1,000bp would be included in the report for all DNA assays.
Terms of Service
Leftover Samples
Leftover samples will be kept for one week after the delivery of results.
Users are welcome to collect any leftover samples during this period, after which the samples will be discarded without further notice.
Please contact our platform specialist for sample pick-up arrangement.
Contact
Mr YIU, Francis
