The BD LSRFortessa™ is a 17-colour analyser with four lasers (Violet 405nm, Blue 488nm, Yellow-Green 561nm and Red 640nm), a fixed optical alignment and a pressure-driven fluidics system. The flow cell is a gel-coupled, and this combined with trigon and octagon detection systems provide increased sensitivity and resolution. This arrangement allows filter and mirror changes within the optical array to be made easily and requires no additional alignment for maximum signal strength, enabling researchers to identify cells with especially dim expression, or quantitate rare cell populations.
Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 40,000 events/second. We recommend a minimum sample volume of 400 μL.
- Annexin V assay
- Cell Cycle Analysis
- Cell Proliferation assay
- Multiplex bead assay for cytokines
LSR Fortessa – Basic Operation Protocol (Tube Mode)
LSR Fortessa – Basic Operation Protocol (HTS Mode)
Staff-in-charge: Ms. YAU, Ka Long (Erica) | 3917 9443