Optical Tweezers/TIRF System Training Workshop – Imaging and Flow Cytometry Core of CPOS

Optical Tweezers/TIRF System Training Workshop

Optical Tweezers/TIRF system is an integrated platform enables users to control, manipulate and observe samples in real time with nanometer precision and femtoNewton resolution. The NanoTracker™ 2 optical tweezers module exerts forces in excess of 100 pN on particles ranging in size from nanometers to microns by using a highly focused 1064nm laser beam, while simultaneously measuring the three-dimensional displacement of the trapped particle with sub-nanometer accuracy and sub-millisecond time resolution. The iLAS2 ring-TIRF Unit with 4 laser lines (405nm, 488nm, 561nm, and 640nm) is also attached to this system for TIRF imaging. Applications include quantitative force measurements, molecular motor mechanics, binding/elasticity of DNA and proteins, cell membrane dynamics and particle uptake into cells, and TIRF Imaging. In this seminar, we will introduce the principle and some applications based on Bruker’s NanoTracker 2 system.

Seminar Session (All are Welcome):

Title: The Application of Optical Tweezers: from Single Molecule to Cells

Speaker: Mr. Youjie Fan, Application Specialist, Bruker

Title: Measuring the Mechanical Properties of Microtubule on Optical Tweezers/TIRF System

Speaker: Mr. Yucheng Ye, PhD Candidate, SBMS, HKUMed

Date: 15 August 2023 (Tuesday)

Time :10:30am – 12:00pm

Venue: Seminar Room 609, A6-09, 6/F, William M.W. Mong Block

Training Session (Registration is required):

* Please be noted that this is part of equipment training, only users who plan to use the equipment within 6 months are suggested to attend the training. After this training, your novice user status will be pending until you complete training of imaging acquisition.


Session Date Time
session 1 15-Aug-2023 13:30 – 15:30
session 2 15-Aug-2023 15:40 – 17:40
session 3 16-Aug-2023 10:00 – 12:00
session 4 16-Aug-2023 13:30 – 15:30
session 5 16-Aug-2023 15:40 – 17:40


Venue: L6-12, Laboratory Block, 21 Sassoon Road

To register, please go to: https://hkuems1.hku.hk/hkuems/ec_hdetail.aspx?ueid=89492

Please refer to the poster below for more detailed information.